Publications

Digital-to-biological converter for on-demand production of biologics.
Boles KS; Kannan K; Gill J; Felderman M; Gouvis H; Hubby B; Kamrud KI; Venter JC and Gibson DG. 2017 Nature Biotechnology 35(7), 672-675.

Design and synthesis of a minimal bacterial genome.
Hutchison CA 3rd; Chuang R-Y; Noskov VN; Assad-Garcia N; Deerinck TJ; Ellisman MH; Gill J; Kannan K; Karas BJ; Ma L; Pelletier JF; Qi Z-Q; Richter AR; Strychalski EA; Sun L; Suzuki Y; Wise KS; Smith HO; Glass JL; Merryman C; Gibson DG and Venter JC. 2016 Science 351(6280), aad6253.

Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.
Suzuki Y; Assad-Garcia N; Kostylev M; Noskov VN; Wise KS; Karas BJ; Stam J; Montague MG; Hanly TJ; Enriquez NJ, Ramon A; Goldgof GM; Richter RA; Vashee S; Chuang R-Y; Winzeler EA; Hutchison CA 3rd; Gibson DG; Smith HO; Glass JI and Venter JC. 2015 Genome Research 25(3): 435-44.

Programming biological operating systems: genome design, assembly and activation.
Gibson DG. 2014. Nature Methods 11(5), 521-6.

Construction of a yeast chromosome.
Gibson DG and Venter JC. 2014 Nature 509(7499), 168-169.

Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG; Glass JI; Latigue C; Noskov VN; Chuang RY; Algire MA; Benders GA; Montague MG; Ma L; Moodie MM; Merryman C; Vashee S; Krishnakumar R; Assad-Garcia N; Andrews-Pfannkoch C; Denisova EA; Young L; Qi ZQ; Segall-Shapiro TH; Calvey CH; Parmer PP; Hutchison CA 3rd; Smith HO and Venter JC. 2010 Science 329(5987) 52-56.

Enzymatic assembly of DNA molecules up to several hundred kilobases.
Gibson DG; Young Li; Chuang R-Y; Venter JC; Hutchison CA 3rd and Smith HO. 2009 Nature Methods 6, 343-345.

Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Lartigue C; Vashee S; Algire MA; Chuang R-Y; Benders GA; Ma L; Noskov VN; Denisova A; Gibson DG; Assad-Garcia N; Alperovich N; Thomas DW; Merryman C; Hutchison CA 3rd; Smith HO; Venter JC and Glass JI 2009 Science 325(5948), 1693-1696.

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome.
Gibson DG; Benders GA; Andrews-Pfannkoch C; Denisova EA.; Baden-Tillson, H; Zaveri, J; Stockwell TB.; Brownley A; Thomas DW; Algire MA; Merryman C; Young L; Noskov VN; Glass JI; Venter JC; Hutchison CA III and Smith HO. 2008 Science 319(5867), 1215-1220.

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Gibson DG; Benders GA; Andrews-Pfannkoch C; Denisova EA; Baden-Tillson H; Zaveri J; Stockwell TB; Brownley A; Thomas DW; Algire MA; Merryman C; Young L; Noskov VN; Glass JI; Venter JC; Hutchison CA 3rd and Smith HO 2008 Science 319(5867), 1215-1220.

Genome transplantation in bacteria: changing one species to another.
Lartigue, C; Glass JI.; Alperovich N; Pieper R; Parmar PP.; Hutchison CA, III; Smith H.O and Venter J.C. 2007 Science 317(5838), 632-638.

Domestication and divergence of Saccharomyces cerevisiae beer yeasts.
Gallone B, Steensels J, Prahl T, Soriaga L, Saels V, Herrera-Malaver B, Merlevede A, Roncoroni M, Voordeckers K, Miraglia L, Teiling C, Steffy B, Taylor M, Schwartz A, Richardson TH, White C, Baele G, Maere S, and Verstrepen K.             2016 Cell 166, 1397-1410.

Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003 (ATCC 4412)
Robson RL; Jones R; Robson M; Schwartz A; Richardson TH 2015 PloS one 10(6), e0127997.

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator.
Ajjawi I; Verruto J; Aqui M; Soriaga LB.; Coppersmith J; Kwok K; Peach L; Orchard E; Kalb R; Xu W; Carlson TJ; Francis K; Konigsfeld K; Bartalis J; Schultz A; Lambert W; Schwartz AS; Brown R and Mollering ER. 2017 Nature Biotechnology 35(7), 647-652.

DNA assembly and cloning in an overnight run with the BioXp 3200 system.
Alvarez CH. 2015 Nature Methods 12(12), v-vi.

DNA library construction using Gibson Assembly.
Thomas S; Maynard ND.; Gill J. 2015 Nature Methods 12(11), i-ii.

Cell-free production of a functional oligomeric form of a Chlamydia major outer-membrane protein (MOMP) for vaccine development.
He W; Felderman M; Evans AC; Geng J; Homan D; Bourguet F; Fischer NO; Li Y; Lam KS; Noy A; Xing L; Cheng HR; Rasley A; Blanchette CD; Kamrud K; Wang N; Gouvis H; Peterson TC; Hubby B and Coleman MA. 2017 Journal of Biological Chemistry 292(36), 15121-15132.

Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination.
Vashee S; Stockwell TB; Alperovich N; Denisova EA; Gibson DG; Cady KC; Miller K; Kannan K; Malouli D; Crawford LB; Voorhies AA; Bruening E; Caposio P and Fruh K. 2017 mSphere 2(5), e00331-17.

Correlation between phenotypic antibiotic susceptibility and the resistome in Pseudomonas aeruginosa.
Jaillard M; van Belkum A; Cady KC; Creely D; Shortridge D; Blanc B; Barbu EM; Dunne W M Jr; Zambardi G; Enright M; Mugnier N; Le Priol C; Schicklin S; Guigon G and Veyrieras JB.      2017 International Journal of Antimicrobial Agents 50(2), 210-218.

Rapidly produced SAM(®) vaccine against H7N9 influenza is immunogenic in mice.
Hekele A; Bertholet S; Archer J; Gibson DG; Palladino G; Brito LA; Otten GR; Brazzoli, M; Buccato S; Bonci A; Casini D; Maione D; Qi Z-Q; Gill JE; Caiazza NC; Urano J; Hubby B; Gao GF; Shu Y; Gregorio ED; Mandl CW; Mason PW; Settembre EC; Ulmer JB; Venter JC; Dormitzer PR; Rappuoli R and Geall, AJ. 2013 Emerging Microbes & Infections 2(8), e52.

Synthetic generation of influenza vaccine viruses for rapid response to pandemics.
Dormitzer PR; Suphaphiphat P; Gibson DG; Wentworth DE; Stockwell TB; Algire MA; Alperovich N; Barro M; Brown DM; Craig S; Dattilo BM; Denisova EA; Souza IDe; Eickmann M; Dugan VG; Ferrari A; Gomila RC; Han L; Judge C; Mane S; Matrosovich M; Merryman C; Palladino G; Palmer GA; Spencer T; Strecker T; Trusheim H; Uhlendorff J; Wen Y; Yee AC; Zaveri J; Zhou B; Becker S; Donabedian A; Mason PW; Glass JI; Rappuoli R and Venter JC 2013 Sci Transl Med 5(185), 185ra168.

Vibrio natriegens as a fast-growing host for molecular biology.
Weinstock, Matthew T.; Hesek, Eric D.; Wilson, Christopher M.; Gibson, Daniel G. 2016 Nature Methods 13(10), 849-851.